Virtual Labs

Gene Stacking – Improved Trait Introduction in Local Cultivars

1. What process introduces foreign DNA into a plant genome?

a: Mutation Explanation

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b: Translation Explanation

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c: Transcription Explanation

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d: Transformation Explanation

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2. Which organism is commonly used for gene transfer in plants?

a: Escherichia coli Explanation

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b: Agrobacterium tumefaciens Explanation

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c: Bacillus subtilis Explanation

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d: Saccharomyces cerevisiae Explanation

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3. What is the role of a promoter in gene expression?

a: Blocks gene transcription Explanation

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b: Initiates transcription Explanation

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c: Ends translation Explanation

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d: Splices RNA Explanation

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4. What type of vector is commonly used in plant genetic engineering?

a: BAC Explanation

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b: Ti plasmid Explanation

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c: Cosmids Explanation

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d: Lambda phage Explanation

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5. What is the role of restriction enzymes in genetic engineering?

a: Join DNA fragments Explanation

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b: Cut DNA at specific sites Explanation

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c: Copy DNA Explanation

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d: Splice RNA Explanation

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6. Which of the following is a constitutive promoter commonly used in plant biotechnology?

a: T7promoter Explanation

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b: lac promoter Explanation

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c: CaMV 35S promoter Explanation

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d: trp promoter Explanation

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7. What is the function of a selectable marker gene in plant transformation?

a: Enhances gene expression Explanation

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b: Allows identification of transformed cells Explanation

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c: Prevents mutation Explanation

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d: Increases recombination Explanation

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8. DNA ligase is essential for:

a: Cutting DNA Explanation

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b: Amplifying DNA Explanation

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c: Joining DNA fragments Explanation

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d: Denaturing DNA Explanation

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9. PCR is primarily used to:

a: Sequence DNA Explanation

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b: Clone proteins Explanation

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c: Amplify DNA Explanation

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d: Modify RNA Explanation

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10. Which plant tissue is most commonly used for regeneration of transgenic plants?

a: Root hairs Explanation

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b: Meristem Explanation

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c: Pollen grains Explanation

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d: Callus Explanation

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